Next-generation sequencing of human mitochondrial reference genomes uncovers high heteroplasmy frequency.

We describe methods for rapid sequencing of the entire human mitochondrial genome (mtgenome), which involve long-range PCR for specific amplification of the mtgenome, pyrosequencing, quantitative mapping of sequence reads to identify sequence variants and heteroplasmy, as well as de novo sequence as...

Celý popis

Uloženo v:
Podrobná bibliografie
Hlavní autoři: Maria Ximena Sosa, I K Ashok Sivakumar, Samantha Maragh, Vamsi Veeramachaneni, Ramesh Hariharan, Minothi Parulekar, Karin M Fredrikson, Timothy T Harkins, Jeffrey Lin, Andrew B Feldman, Pramila Tata, Georg B Ehret, Aravinda Chakravarti
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science (PLoS) 2012-01-01
Edice:PLoS Computational Biology
On-line přístup:http://europepmc.org/articles/PMC3486893?pdf=render
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky