Steady-State Kinetics of Enzyme-Catalyzed Hydrolysis of Echothiophate, a P–S Bonded Organophosphorus as Monitored by Spectrofluorimetry

Enzyme-catalyzed hydrolysis of echothiophate, a P−S bonded organophosphorus (OP) model, was spectrofluorimetrically monitored, using Calbiochem Probe IV as the thiol reagent. OP hydrolases were: the G117H mutant of human butyrylcholinesterase capable of hydrolyzing OPs, and a multiple muta...

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Detalhes bibliográficos
Main Authors: Irina V. Zueva, Sofya V. Lushchekina, David Daudé, Eric Chabrière, Patrick Masson
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2020-03-01
Colecção:Molecules
Assuntos:
p–s bonded organophosphorus agents
echothiophate
calbiochem probe iv
organophosphate hydrolase
phosphotriesterase
cholinesterase
qm/mm
Acesso em linha:https://www.mdpi.com/1420-3049/25/6/1371
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