Expression and purification of ELP-intein-tagged target proteins in high cell density <it>E. coli </it>fermentation
<p>Abstract</p> <p>Background</p> <p>Elastin-like polypeptides (ELPs) are useful tools that can be used to non-chromatographically purify proteins. When paired with self-cleaving inteins, they can be used as economical self-cleaving purification tags. However, ELPs and ELP-tagged target proteins hav...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
BMC
2010-10-01
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| Colección: | Microbial Cell Factories |
| Acceso en línea: | http://www.microbialcellfactories.com/content/9/1/77 |
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