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Expression and purification of ELP-intein-tagged target proteins in high cell density <it>E. coli </it>fermentation

<p>Abstract</p> <p>Background</p> <p>Elastin-like polypeptides (ELPs) are useful tools that can be used to non-chromatographically purify proteins. When paired with self-cleaving inteins, they can be used as economical self-cleaving purification tags. However, ELPs and ELP-tagged target proteins hav...

Бүрэн тодорхойлолт

-д хадгалсан:
Номзүйн дэлгэрэнгүй
Үндсэн зохиолчид: Wood David W, Fong Baley A
Формат: Artigo
Хэл сонгох:Inglês
Хэвлэсэн: BMC 2010-10-01
Цуврал:Microbial Cell Factories
Онлайн хандалт:http://www.microbialcellfactories.com/content/9/1/77
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