Application of DNA Topoisomerase-Activated Adapters to Riboprobe Synthesis
Topoisomerase-activated adapters for rapid incorporation of the T7 promoter into PCR products were made by hybridizing synthetic oligonucleotides and activating vaccinia DNA topoisomerase I. The adapters were used to incorporate the T7 promoter sequence into PCR products amplified from cDNA and geno...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis Group
2000-06-01
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| coleção: | BioTechniques |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/00286st06 |
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