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Expansion microscopy of C. elegans

We recently developed expansion microscopy (ExM), which achieves nanoscale-precise imaging of specimens at ~70 nm resolution (with ~4.5x linear expansion) by isotropic swelling of chemically processed, hydrogel-embedded tissue. ExM of C. elegans is challenged by its cuticle, which is stiff and imper...

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Principais autores: Chih-Chieh (Jay) Yu, Nicholas C Barry, Asmamaw T Wassie, Anubhav Sinha, Abhishek Bhattacharya, Shoh Asano, Chi Zhang, Fei Chen, Oliver Hobert, Miriam B Goodman, Gal Haspel, Edward S Boyden
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications Ltd 2020-05-01
coleção:eLife
Assuntos:
expansion microscopy
super-resolution imaging
immunohistochemistry
in situ hybridization
synapses
gene expression
Acesso em linha:https://elifesciences.org/articles/46249
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