Engineering Adenine Deaminase TadA for Precise and PAM‐Flexible Point Mutagenesis and Gradient‐Tuning Endogenous Protein Design

Abstract Base editing enables precise nucleotide substitutions within a relatively broad editing window (5–6 nucleotides). However, considerable bystander editing significantly compromise its accuracy. Point mutagenesis, a powerful approach for gradient‐tuning protein function, facilitates the gener...

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Hlavní autoři:	Kangli Sun, Si Cheng, Nan Chai, Jianing Mi, Ruixiang Zhang, Qian Qian, Zhiye Zheng, Ke Chen, Dongchang Zeng, Xin Peng, Mengyuan Shen, Degui Zhou, Qinlong Zhu, Qi Liu, Jiantao Tan
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Wiley 2025-09-01
Edice:	Advanced Science
Témata:	Badh2 gradient‐tuned protein design point mutagenesis precise base editing TadA variants
On-line přístup:	https://doi.org/10.1002/advs.202506644
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