Engineering Adenine Deaminase TadA for Precise and PAM‐Flexible Point Mutagenesis and Gradient‐Tuning Endogenous Protein Design

Abstract Base editing enables precise nucleotide substitutions within a relatively broad editing window (5–6 nucleotides). However, considerable bystander editing significantly compromise its accuracy. Point mutagenesis, a powerful approach for gradient‐tuning protein function, facilitates the gener...

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Principais autores:	Kangli Sun, Si Cheng, Nan Chai, Jianing Mi, Ruixiang Zhang, Qian Qian, Zhiye Zheng, Ke Chen, Dongchang Zeng, Xin Peng, Mengyuan Shen, Degui Zhou, Qinlong Zhu, Qi Liu, Jiantao Tan
Formato:	Artigo
Idioma:	Inglês
Publicado:	Wiley 2025-09-01
Series:	Advanced Science
Assuntos:	Badh2 gradient‐tuned protein design point mutagenesis precise base editing TadA variants
Acceso en liña:	https://doi.org/10.1002/advs.202506644
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