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Sensitive Detection of 8-Nitroguanine in DNA by Chemical Derivatization Coupled with Online Solid-Phase Extraction LC-MS/MS

8-Nitroguanine (8-nitroG) is a major mutagenic nucleobase lesion generated by peroxynitrite during inflammation and has been used as a potential biomarker to evaluate inflammation-related carcinogenesis. Here, we present an online solid-phase extraction (SPE) LC-MS/MS method with 6-methoxy-2-naphthy...

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Principais autores: Chiung-Wen Hu, Yuan-Jhe Chang, Jian-Lian Chen, Yu-Wen Hsu, Mu-Rong Chao
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2018-03-01
coleção:Molecules
Assuntos:
online solid-phase extraction
LC-MS/MS
peroxynitrite
nitrated DNA lesion
derivatization
isotope-dilution
Acesso em linha:http://www.mdpi.com/1420-3049/23/3/605
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