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Secretory expression of cyclohexanone monooxygenase by methylotrophic yeast for efficient omeprazole sulfide bio-oxidation

Abstract Prochiral pyrmetazole can be asymmetrically oxidized into (S)-omeprazole, a proton pump inhibitor that is used to treat gastroesophageal reflux, by an engineered cyclohexanone monooxygenase (CHMO Acineto -Mut) that has high stereoselectivity. CHMO Acineto -Mut is produced by heterologous ex...

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Principais autores: Ya-Jing Li, Yu-Cong Zheng, Qiang Geng, Feng Liu, Zhi-Jun Zhang, Jian-He Xu, Hui-Lei Yu
Format: Artigo
Sprog:Inglês
Udgivet: SpringerOpen 2021-08-01
Serier:Bioresources and Bioprocessing
Fag:
Cyclohexanone monooxygenase
Omeprazole sulfoxide
Pichia pastoris
Secretory expression
Asymmetric oxidation
Online adgang:https://doi.org/10.1186/s40643-021-00430-1
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