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Optimized nuclei isolation and snRNA-seq reveal oligodendrocyte pathway dysregulation in MOGHE brain tissue from pediatric patients

Abstract Single-cell RNA sequencing (scRNA-seq) is a powerful tool for exploring cellular diversity, but isolating intact cells from complex tissues like the brain remains challenging. Single-nucleus RNA sequencing (snRNA-seq) overcomes these limitations by profiling nuclear RNA from frozen or archi...

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Principais autores: Clara Tuccari di San Carlo, Roberto Semeraro, Valentina Cetica, Dalila De Vita, Benedetta Mazzinghi, Antonio Falace, Cristiana Pelorosso, Paola Romagnani, Alberto Magi, Renzo Guerrini, Valerio Conti
Format: Artigo
Sprog:Inglês
Udgivet: Nature Portfolio 2026-05-01
Serier:Scientific Reports
Online adgang:https://doi.org/10.1038/s41598-026-54112-z
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