In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration

Abstract Fluorescent staining of newly transcribed RNA via metabolic labelling with 5-ethynyluridine (EU) and click chemistry enables visualisation of changes in transcription, such as in conditions of cellular stress. Here, we tested whether EU labelling can be used to examine transcription in vivo...

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Principais autores:	Lisanne J. van’t Sant, Joshua J. White, Jan H. J. Hoeijmakers, Wilbert P. Vermeij, Dick Jaarsma
Format:	Artigo
Jezik:	Inglês
Izdano:	BMC 2021-05-01
Serija:	Acta Neuropathologica Communications
Teme:	Purkinje cell degeneration 5-Ethynylurdine Nascent RNA Transcription stress DNA damage Ercc1
Online dostop:	https://doi.org/10.1186/s40478-021-01200-y
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