Isolation of the Sarcoplasmic Reticulum Ca²⁺-ATPase from Rabbit Fast-Twitch Muscle

The sarcoendoplasmic reticulum Ca²⁺-ATPase (SERCA) is a membrane protein that is destabilized during purification in the absence of calcium ions. The disaccharide trehalose is a protein stabilizer that accumulates in the yeast cytoplasm when under stress. In the present work, SERCA was pu...

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Principais autores:	Miguel A. Rivera-Morán, José G. Sampedro
Formato:	Artigo
Idioma:	Inglês
Publicado em:	MDPI AG 2023-10-01
coleção:	Methods and Protocols
Assuntos:	SERCA Ca<sup>2+</sup>-ATPase trehalose enzyme stabilization fluorescent labeling ATP binding
Acesso em linha:	https://www.mdpi.com/2409-9279/6/5/102
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