Protocol for measuring beta-secretase activity in cell and tissue lysate using a fluorogenic peptide substrate in a 96-well format
Summary: The protease β-secretase (BACE1) plays a crucial role in the formation of amyloid-beta peptides. Here, we present a protocol for real-time quantification of BACE1 activity in brain tissue and cell lysates using a fluorogenic peptide substrate in a 96-well format. We describe steps for reage...
Kaydedildi:
| Asıl Yazarlar: | , , , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Elsevier
2025-06-01
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| Seri Bilgileri: | STAR Protocols |
| Konular: | |
| Online Erişim: | http://www.sciencedirect.com/science/article/pii/S2666166725002916 |
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