Protocol for live-cell super-resolution imaging of transport of pre-ribosomal subunits through the nuclear pore complex
Summary: Here, we present a protocol for single-molecule super-resolution imaging of the nuclear export of pre-ribosomal subunits pre-40S and pre-60S through nuclear pore complexes. We describe steps for plating cells and co-transfecting cells. We then detail steps for using single-point edge-excita...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Elsevier
2024-03-01
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| Collection: | STAR Protocols |
| Sujets: | |
| Accès en ligne: | http://www.sciencedirect.com/science/article/pii/S2666166723007578 |
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