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Ecological Observations Based on Functional Gene Sequencing Are Sensitive to the Amplicon Processing Method

ABSTRACT Until recently, the de facto method for short-read-based amplicon reconstruction was a sequence similarity threshold approach (operational taxonomic units [OTUs]). This has changed with the amplicon sequence variant (ASV) method where distributions are fitted to abundance profiles of indivi...

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Principais autores: Fabien Cholet, Agata Lisik, Hélène Agogué, Umer Z. Ijaz, Philippe Pineau, Nicolas Lachaussée, Cindy J. Smith
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2022-08-01
叢編:mSphere
主題:
amplicon sequencing
functional genes
nitrogen cycle
amplicon sequence variant
coastal sediments
在線閱讀:https://journals.asm.org/doi/10.1128/msphere.00324-22
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