Ecological Observations Based on Functional Gene Sequencing Are Sensitive to the Amplicon Processing Method

ABSTRACT Until recently, the de facto method for short-read-based amplicon reconstruction was a sequence similarity threshold approach (operational taxonomic units [OTUs]). This has changed with the amplicon sequence variant (ASV) method where distributions are fitted to abundance profiles of indivi...

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Autori principali:	Fabien Cholet, Agata Lisik, Hélène Agogué, Umer Z. Ijaz, Philippe Pineau, Nicolas Lachaussée, Cindy J. Smith
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology 2022-08-01
Serie:	mSphere
Soggetti:	amplicon sequencing functional genes nitrogen cycle amplicon sequence variant coastal sediments
Accesso online:	https://journals.asm.org/doi/10.1128/msphere.00324-22
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