Variation in guide RNA library representation results in gene effect score bias in genome-wide CRISPR screens

Abstract Genome wide CRISPR-based perturbation screens are powerful discovery tools enabling the identification of novel gene dependencies through either gain or loss of function. While genome wide guide RNA (gRNA) libraries have advantages when using enAsCas12a, such as multiplex single gRNAs per g...

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Autori principali:	Paul Metz, Sofia Alves-Vasconcelos, Richard Wallbank, Joey Riepsaame, Stuart Brown, A. Bassim Hassan
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	BMC 2026-02-01
Serie:	BMC Genomics
Soggetti:	CRISPR Enhanced AsCas12a SgRNA Human plasma like media Perturbation screen
Accesso online:	https://doi.org/10.1186/s12864-026-12658-2
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