Mouse kidney nuclear isolation and library preparation for single-cell combinatorial indexing RNA sequencing

Summary: Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq3) enables high-throughput single-nucleus transcriptomic profiling of multiple samples in one experiment. Here, we describe an optimized protocol of mouse kidney nuclei isolation and sci-RNA-seq3 library preparation. The use of a...

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Detalles Bibliográficos

Principais autores:	Haikuo Li, Benjamin D. Humphreys
Formato:	Artigo
Idioma:	Inglês
Publicado:	Elsevier 2022-12-01
Series:	STAR Protocols
Assuntos:	Cell Biology Cell isolation Model Organisms Molecular Biology RNAseq Single Cell
Acceso en liña:	http://www.sciencedirect.com/science/article/pii/S2666166722007845
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