Optimized protocol for mouse footpad immune cell isolation for single-cell RNA sequencing and flow cytometry

Summary: Single-cell RNA sequencing (scRNA-seq) requires the preparation of a highly viable single-cell suspension to get reliable sequencing results. Here, we present a protocol for isolating mouse footpad leukocytes while maintaining high viability. We describe steps for footpad collection, enzyma...

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Hlavní autoři:	Muddassar Hameed, Pallavi Rai, Melissa Makris, James Weger-Lucarelli
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2023-09-01
Edice:	STAR Protocols
Témata:	Cell Biology Cell isolation Single Cell Flow Cytometry/Mass Cytometry RNAseq Immunology
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S2666166723003763
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