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High coenzyme affinity chimeric amine dehydrogenase based on domain engineering

Abstract NADH-dependent phenylalanine amine dehydrogenase (F-AmDH) engineered from phenylalanine dehydrogenase (PheDH) catalyzes the synthesis of aromatic chiral amines from prochiral ketone substrates. However, its low coenzyme affinity and catalytic efficiency limit its industrial application. Her...

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Principais autores: Jialin Li, Xiaoqing Mu, Tao Wu, Yan Xu
Format: Artigo
Sprog:Inglês
Udgivet: SpringerOpen 2022-03-01
Serier:Bioresources and Bioprocessing
Fag:
Amine dehydrogenase
Coenzyme affinity
Coenzyme binding domain
Catalytic efficiency
Online adgang:https://doi.org/10.1186/s40643-022-00528-0
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