High-accuracy crRNA array assembly strategy for multiplex CRISPR

Simultaneous targeting of multiple loci with the CRISPR system, a tool known as multiplex CRISPR, offers greater feasibility for manipulating and elucidating the intricate and redundant endogenous networks underlying complex cellular functions. Owing to the versatility of continuously emerging Cas n...

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Detalles Bibliográficos

Principais autores:	Xiangtong Zhao, Lixian Yang, Peng Li, Zijing Cheng, Yongshi Jia, Limin Luo, Aihong Bi, Hanchu Xiong, Haibo Zhang, Hongen Xu, Jinrui Zhang, Yaodong Zhang
Formato:	Artigo
Idioma:	Inglês
Publicado:	Elsevier 2025-03-01
Series:	Molecular Therapy: Nucleic Acids
Assuntos:	MT: RNA/DNA Editing multiplex CRISPR crRNA CRISPR array Golden Gate Assembly AsCas12a
Acceso en liña:	http://www.sciencedirect.com/science/article/pii/S2162253124003159
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