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High-accuracy crRNA array assembly strategy for multiplex CRISPR

Simultaneous targeting of multiple loci with the CRISPR system, a tool known as multiplex CRISPR, offers greater feasibility for manipulating and elucidating the intricate and redundant endogenous networks underlying complex cellular functions. Owing to the versatility of continuously emerging Cas n...

Ful tanımlama

Kaydedildi:
Asıl Yazarlar: Xiangtong Zhao, Lixian Yang, Peng Li, Zijing Cheng, Yongshi Jia, Limin Luo, Aihong Bi, Hanchu Xiong, Haibo Zhang, Hongen Xu, Jinrui Zhang, Yaodong Zhang
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Elsevier 2025-03-01
Seri Bilgileri:Molecular Therapy: Nucleic Acids
Konular:
MT: RNA/DNA Editing
multiplex CRISPR
crRNA
CRISPR array
Golden Gate Assembly
AsCas12a
Online Erişim:http://www.sciencedirect.com/science/article/pii/S2162253124003159
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