Scalable production and purification of engineered ARRDC1-mediated microvesicles in a HEK293 suspension cell system

Abstract Engineering of human ARRDC1-mediated microvesicles (ARMMs) as non-viral vehicles for delivery of gene therapies bears the potential to enable novel therapeutic paradigms. We evaluated two scalable strategies to generate ARMMs loaded with protein cargo, by transient transfection or stable ce...

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Detalhes bibliográficos

Principais autores:	Kristin Luther, Ali Navaei, Leah Gens, Carson Semple, Pearl Moharil, Ilaria Passalacqua, Komal Vyas, Qiyu Wang, Shu-Lin Liu, Lucy Sun, Senthil Ramaswamy, Davide Zocco, Joseph F. Nabhan
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Nature Portfolio 2025-03-01
coleção:	Scientific Reports
Assuntos:	Extracellular vesicles ARRDC1-mediated microvesicles ARMMs 5B8 Transient transfection Stable producer cell line
Acesso em linha:	https://doi.org/10.1038/s41598-025-87674-5
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