Reverse-phase chromatography removes double-stranded RNA, fragments, and residual template to decrease immunogenicity and increase cell potency of mRNA and saRNA

mRNA is produced by in vitro transcription reaction, which also leads to formation of immuno-stimulatory impurities, such as double-stranded RNA (dsRNA). dsRNA leads to activation of innate immune response linked to inhibition of protein synthesis. Its removal from mRNA preparations increases effici...

وصف كامل

التفاصيل البيبلوغرافية

المؤلفون الرئيسيون:	Andreja Krušič, Nina Mencin, Marta Leban, Evelin Nett, Mario Perković, Ugur Sahin, Polona Megušar, Aleš Štrancar, Rok Sekirnik
التنسيق:	Artigo
اللغة:	Inglês
منشور في:	Elsevier 2025-06-01
سلاسل:	Molecular Therapy: Nucleic Acids
الموضوعات:	MT: Delivery Strategies dsRNA vaccines HPLC chromatography mRNA
الوصول للمادة أونلاين:	http://www.sciencedirect.com/science/article/pii/S2162253125000459
الوسوم:	إضافة وسم لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!