Reverse-phase chromatography removes double-stranded RNA, fragments, and residual template to decrease immunogenicity and increase cell potency of mRNA and saRNA

mRNA is produced by in vitro transcription reaction, which also leads to formation of immuno-stimulatory impurities, such as double-stranded RNA (dsRNA). dsRNA leads to activation of innate immune response linked to inhibition of protein synthesis. Its removal from mRNA preparations increases effici...

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Bibliografski detalji

Glavni autori:	Andreja Krušič, Nina Mencin, Marta Leban, Evelin Nett, Mario Perković, Ugur Sahin, Polona Megušar, Aleš Štrancar, Rok Sekirnik
Format:	Artigo
Jezik:	Inglês
Izdano:	Elsevier 2025-06-01
Serija:	Molecular Therapy: Nucleic Acids
Teme:	MT: Delivery Strategies dsRNA vaccines HPLC chromatography mRNA
Online pristup:	http://www.sciencedirect.com/science/article/pii/S2162253125000459
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