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Prime editing using paired pegRNAs targeting NG- or NGG-PAM in rice

Prime editing (PE) enables precise genome modification, i.e., all 12 types of base substitution, as well as designed insertion and deletion. Previously, we developed an efficient PE system using a pair of engineered pegRNAs (epegRNAs), appending an RNA pseudoknot sequence to the 3′ends of pegRNAs to...

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書誌詳細
主要な著者: Ayako Nishizawa-Yokoi, Keiko Iida, Akiko Mori, Seiichi Toki
フォーマット: Artigo
言語:Inglês
出版事項: Frontiers Media S.A. 2025-08-01
シリーズ:Frontiers in Genome Editing
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オンライン・アクセス:https://www.frontiersin.org/articles/10.3389/fgeed.2025.1550308/full
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