CRISPR-based large-scale modeling of loss-of-function mutations to investigate mechanisms of stress resistance in cancer

Summary: Dissecting mechanisms driving subclone expansion in primary cancers has been challenging. Here, we present a protocol to systematically disrupt entire gene networks and assess the functional impact of this perturbation on cancer cell fitness. By combining arrayed CRISPR libraries and high-c...

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Detalhes bibliográficos

Principais autores:	Fabrizio Simeoni, Ioannis Loukas, Thomas Stuart Wilson, Paola Scaffidi
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2023-03-01
coleção:	STAR Protocols
Assuntos:	Cell Biology Cell-based Assays Cancer Genetics High-throughput Screening Molecular Biology
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166723000552
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