Optimized protocol for tRNA identification in the ribosomal complexes from human cell lines
Summary: Here, we describe a protocol for tRNA identification in the 60S ribosome-nascent peptide complex co-purified with Nuclear Export Mediator Factor (NEMF), a responsible factor for C-terminal alanine and threonine tailing of the nascent peptide. Our protocol is based on regular reverse transcr...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Elsevier
2021-09-01
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| Серии: | STAR Protocols |
| Предметы: | |
| Online-ссылка: | http://www.sciencedirect.com/science/article/pii/S2666166721003221 |
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