Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis

Abstract Background Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in multiplex genome editing technologies. However, simultaneously modulating multiple genes on the chromosome remains ch...

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Bibliografische gegevens

Hoofdauteurs:	Dingyu Liu, Can Huang, Jiaxin Guo, Peiji Zhang, Tao Chen, Zhiwen Wang, Xueming Zhao
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	BMC 2019-09-01
Reeks:	Biotechnology for Biofuels
Onderwerpen:	CRISPR/Cas9n Multiplex genome editing Combinatorial metabolic engineering Riboflavin Nick repair mechanism
Online toegang:	http://link.springer.com/article/10.1186/s13068-019-1537-1
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