Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis

Abstract Background Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in multiplex genome editing technologies. However, simultaneously modulating multiple genes on the chromosome remains ch...

ver descrição completa

Detalhes bibliográficos

Principais autores:	Dingyu Liu, Can Huang, Jiaxin Guo, Peiji Zhang, Tao Chen, Zhiwen Wang, Xueming Zhao
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2019-09-01
coleção:	Biotechnology for Biofuels
Assuntos:	CRISPR/Cas9n Multiplex genome editing Combinatorial metabolic engineering Riboflavin Nick repair mechanism
Acesso em linha:	http://link.springer.com/article/10.1186/s13068-019-1537-1
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!