Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis

The simultaneous sequencing of samples from multiple individuals increases the efficiency of next-generation sequencing (NGS) while also reducing costs. Here we describe a novel and simple approach for sequencing DNA from multiple individuals per barcode. Our strategy relies on the endonuclease dige...

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Hauptverfasser:	Jeppe D. Andersen, Vania Pereira, Carlotta Pietroni, Martin Mikkelsen, Peter Johansen, Claus Børsting, Niels Morling
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Taylor & Francis Group 2014-08-01
Schriftenreihe:	BioTechniques
Schlagworte:	next-generation sequencing restriction endonucleases DNA library preparation amplicons melanocortin 1 receptor (MC1R)
Online-Zugang:	https://www.future-science.com/doi/10.2144/000114200
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