Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis

The simultaneous sequencing of samples from multiple individuals increases the efficiency of next-generation sequencing (NGS) while also reducing costs. Here we describe a novel and simple approach for sequencing DNA from multiple individuals per barcode. Our strategy relies on the endonuclease dige...

Cur síos iomlán

Sonraí bibleagrafaíochta

Príomhchruthaitheoirí:	Jeppe D. Andersen, Vania Pereira, Carlotta Pietroni, Martin Mikkelsen, Peter Johansen, Claus Børsting, Niels Morling
Formáid:	Artigo
Teanga:	Inglês
Foilsithe / Cruthaithe:	Taylor & Francis Group 2014-08-01
Sraith:	BioTechniques
Ábhair:	next-generation sequencing restriction endonucleases DNA library preparation amplicons melanocortin 1 receptor (MC1R)
Rochtain ar líne:	https://www.future-science.com/doi/10.2144/000114200
Clibeanna:	Cuir clib leis Níl clibeanna ann, Bí ar an gcéad duine le clib a chur leis an taifead seo!