Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis

The simultaneous sequencing of samples from multiple individuals increases the efficiency of next-generation sequencing (NGS) while also reducing costs. Here we describe a novel and simple approach for sequencing DNA from multiple individuals per barcode. Our strategy relies on the endonuclease dige...

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Detalhes bibliográficos
Main Authors: Jeppe D. Andersen, Vania Pereira, Carlotta Pietroni, Martin Mikkelsen, Peter Johansen, Claus Børsting, Niels Morling
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis Group 2014-08-01
Colecção:BioTechniques
Assuntos:
next-generation sequencing
restriction endonucleases
DNA library preparation
amplicons
melanocortin 1 receptor (MC1R)
Acesso em linha:https://www.future-science.com/doi/10.2144/000114200
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