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New Assay for Quantification of PEGylated Proteins During in Vitro Permeability Studies

One of the major challenges when analyzing very low amounts of PEGylated proteins is finding a sensitive analytical method. Immunoassays are most frequently used, however, conjugation can partially or completely mask protein epitopes, which can substantially lower the response and influence the qua...

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Principais autores: Špela Jalen, Vanja Smilović, Katarina Fidler, Barbara Podobnik, Maja Marušić, Radovan Komel, Vladka Gaberc-Porekar, Simona Jevševar
Format: Artigo
Sprog:Inglês
Udgivet: Slovenian Chemical Society 2014-09-01
Serier:Acta Chimica Slovenica
Fag:
Immunoassay
PEGylation
His-tag
Biotin
TNF-α
Caco-2
Online adgang:https://acsi-journal.eu/index.php/ACSi/article/view/211
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