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Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays

Summary: Hematopoietic stem and progenitor cells (HSPCs) reside in the bone marrow and supply blood cells. Efficient methods for isolation of HSPCs are required. Here, we present protocols for the isolation of human and murine HSPCs using manual and FACS-assisted techniques. Isolated HSPCs can be us...

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Principais autores: Alfredo Rodríguez, Jessica Filiatrault, Patricia Flores-Guzmán, Héctor Mayani, Kalindi Parmar, Alan D. D’Andrea
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021-12-01
Serier:STAR Protocols
Fag:
Cell Biology
Cell isolation
Flow Cytometry/Mass Cytometry
Cell-based Assays
Genetics
Microscopy
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166721005529
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