Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays

Summary: Hematopoietic stem and progenitor cells (HSPCs) reside in the bone marrow and supply blood cells. Efficient methods for isolation of HSPCs are required. Here, we present protocols for the isolation of human and murine HSPCs using manual and FACS-assisted techniques. Isolated HSPCs can be us...

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Autors principals:	Alfredo Rodríguez, Jessica Filiatrault, Patricia Flores-Guzmán, Héctor Mayani, Kalindi Parmar, Alan D. D’Andrea
Format:	Artigo
Idioma:	Inglês
Publicat:	Elsevier 2021-12-01
Col·lecció:	STAR Protocols
Matèries:	Cell Biology Cell isolation Flow Cytometry/Mass Cytometry Cell-based Assays Genetics Microscopy
Accés en línia:	http://www.sciencedirect.com/science/article/pii/S2666166721005529
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