Controlled Hot Start and Improved Specificity in Carrying Out PCR Utilizing Touch-Up and Loop Incorporated Primers (TULIPS)
The PCR technique often yields nonspecific products. To overcome this problem, a simple, specific and efficient method was designed: touch-up and loop incorporated primers (TULIPS)-PCR. This approach utilizes loop primers (i.e., additional nontemplate 5′sequence that self-anneals to the 3′ region an...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Taylor & Francis Group
2000-11-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00295st03 |
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