BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI-absence is applicable as a universal transformation platform for wild-type Bacillus

Abstract Background To effectively introduce plasmids into Bacillus species and conduct genetic manipulations in Bacillus chassis strains, it is essential to optimize transformation methods. These methods aim to extend the period of competence and enhance the permeability of the cell membrane to fac...

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Bibliografische gegevens

Hoofdauteurs:	Zhao Xingya, Fu Xiaoping, Zhen Jie, Yang Jun, Zheng Hongchen, Bai Wenqin, Song Hui
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	BMC 2024-08-01
Reeks:	Microbial Cell Factories
Onderwerpen:	Plasmid transformation Restriction-modification system Regulatory mechanism Bacillus species
Online toegang:	https://doi.org/10.1186/s12934-024-02493-z
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