pET expression vector customized for efficient seamless cloning

Here we present a modification of the widely used pET29 expression vector for use in rapid and straightforward parallel cloning via a gene replacement and Golden Gate strategy. The modification can be applied to other expression vectors for Gram-negative bacteria. We have used the modified vectors t...

Cijeli opis

Bibliografski detalji

Glavni autori:	Dragana Dobrijevic, Lily A Nematollahi, Helen C Hailes, John M Ward
Format:	Artigo
Jezik:	Inglês
Izdano:	Taylor & Francis Group 2020-11-01
Serija:	BioTechniques
Teme:	expression in E. coli Golden Gate pET vectors sacB seamless cloning
Online pristup:	https://www.future-science.com/doi/10.2144/btn-2020-0101
Oznake:	Dodaj oznaku Bez oznaka, Budi prvi tko označuje ovaj zapis!