Optimizing T4 DNA polymerase conditions enhances the efficiency of one-step sequence- and ligation-independent cloning

Previously, we developed a one-step sequence- and ligation-independent cloning (SLIC) method that is simple, fast, and cost-effective. However, although one-step SLIC generally works well, its cloning efficiency is occasionally poor, potentially due to formation of stable secondary structures within...

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Autors principals:	Mohammad Nazrul Islam, Kyeong Won Lee, Hyung-Soon Yim, Seong Hyuk Lee, Hae Chang Jung, Jung-Hyun Lee, Jae-Yeon Jeong
Format:	Artigo
Idioma:	Inglês
Publicat:	Taylor & Francis Group 2017-09-01
Col·lecció:	BioTechniques
Matèries:	seamless cloning SLIC T4 DNA polymerase
Accés en línia:	https://www.future-science.com/doi/10.2144/000114588
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