Caveats of Using Overexpression Approaches to Screen Cellular Host IFITM Proteins for Antiviral Activity

Ectopic protein overexpression in immortalised cell lines is a commonly used method to screen host factors for their antiviral activity against different viruses. However, the question remains as to what extent such artificial protein overexpression recapitulates endogenous protein function. Previou...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Tina Meischel, Svenja Fritzlar, Fernando Villalón-Letelier, Jeffrey M. Smith, Andrew G. Brooks, Patrick C. Reading, Sarah L. Londrigan
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	MDPI AG 2023-03-01
Schriftenreihe:	Pathogens
Schlagworte:	respiratory viruses innate immunity IFITM proteins host antiviral factors influenza A virus parainfluenza virus
Online-Zugang:	https://www.mdpi.com/2076-0817/12/4/519
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!