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Development of a Bicistronic Vector for the Expression of a CRISPR/Cas9-mCherry System in Fish Cell Lines

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been widely used in animals as an efficient genome editing tool. In fish cells, the technique has been difficult to implement due to the lack of proper vectors that use active promoters...

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Bibliografske podrobnosti
Principais autores: Sebastian Escobar-Aguirre, Duxan Arancibia, Amanda Escorza, Cristián Bravo, María Estela Andrés, Pedro Zamorano, Víctor Martínez
Format: Artigo
Jezik:Inglês
Izdano: MDPI AG 2019-01-01
Serija:Cells
Teme:
Online dostop:https://www.mdpi.com/2073-4409/8/1/75
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