Development of a Bicistronic Vector for the Expression of a CRISPR/Cas9-mCherry System in Fish Cell Lines

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been widely used in animals as an efficient genome editing tool. In fish cells, the technique has been difficult to implement due to the lack of proper vectors that use active promoters...

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Hlavní autoři:	Sebastian Escobar-Aguirre, Duxan Arancibia, Amanda Escorza, Cristián Bravo, María Estela Andrés, Pedro Zamorano, Víctor Martínez
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	MDPI AG 2019-01-01
Edice:	Cells
Témata:	CRISPR/Cas9 U6 promoter fish cells
On-line přístup:	https://www.mdpi.com/2073-4409/8/1/75
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