A recurrent sequencing artifact on Illumina sequencers with two-color fluorescent dye chemistry and its impact on somatic variant detection

Abstract Background The sequencing-by-synthesis technology by Illumina, Inc. enables efficient and scalable readouts of mutations from genomic data. To enhance sequencing speed and efficiency, Illumina has shifted from the four-color base calling chemistry of the HiSeq series to a two-color fluoresc...

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Hauptverfasser:	Beverly J. Fu, Vinayak V. Viswanadham, Dominika Maziec, Hu Jin, Peter J. Park
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	BMC 2026-04-01
Schriftenreihe:	Genome Biology
Schlagworte:	Next-generation sequencing Illumina NovaSeq 6000 Somatic mutations Mosaic mutations Sequencing artifacts
Online-Zugang:	https://doi.org/10.1186/s13059-026-04081-3
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