An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum

Abstract PfAgo, a thermophilic Argonaute nuclease from Pyrococcus furiosus, is widely used in various fields due to its high DNA‐guided DNA cleavage activity. However, its high‐temperature‐dependent cleavage activity largely restricts its applications in moderate‐temperature scenarios. In this study...

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Detalhes bibliográficos

Principais autores:	Longyu Wang, Xiaochen Xie, Fuyong Huang, Qiang Wei, Tianxin Cai, Na Yu, Shi Chen, Fei Wang, Wanping Chen, Chin‐yu Chen, Chunhua Li, Lixin Ma
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Wiley 2025-08-01
Colecção:	Advanced Science
Assuntos:	cold adaptation nucleases PfAgo rational engineering thermophilic archaea
Acesso em linha:	https://doi.org/10.1002/advs.202416631
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