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Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics

Investigation of human CNS disease and drug effects has been hampered by the lack of a system that enables single-cell analysis of live adult patient brain cells. We developed a culturing system, based on a papain-aided procedure, for resected adult human brain tissue removed during neurosurgery. We...

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Principais autores: Jennifer M. Spaethling, Young-Ji Na, Jaehee Lee, Alexandra V. Ulyanova, Gordon H. Baltuch, Thomas J. Bell, Steven Brem, H. Isaac Chen, Hannah Dueck, Stephen A. Fisher, Marcela P. Garcia, Mugdha Khaladkar, David K. Kung, Timothy H. Lucas Jr., Donald M. O’Rourke, Derek Stefanik, Jinhui Wang, John A. Wolf, Tamas Bartfai, M. Sean Grady, Jai-Yoon Sul, Junhyong Kim, James H. Eberwine
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2017-01-01
Serier:Cell Reports
Fag:
single cell
human
primary cell
adult
aged
neuron
astrocyte
microglia
endothelial cell
transcriptome
Online adgang:http://www.sciencedirect.com/science/article/pii/S2211124716317739
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