Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells
Summary: DNA end resection converts broken ends of double-stranded DNA (dsDNA) to 3′-single-stranded DNA (3′-ssDNA). The extent of resection regulates DNA double-strand break (DSB) repair pathway choice and thereby genomic stability. Here, we characterize an optimized immunofluorescence (IF) microsc...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2021-12-01
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| Col·lecció: | STAR Protocols |
| Matèries: | |
| Accés en línia: | http://www.sciencedirect.com/science/article/pii/S2666166721006845 |
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