Protocol to extract actively translated mRNAs from mouse hypothalamus by translating ribosome affinity purification

Summary: Here, we present an in-depth protocol for extracting ribosome-bound mRNAs in low-abundance cells of hypothalamic nuclei. mRNAs are extracted from the micropunched tissue using refined translating ribosome affinity purification. Isolated RNAs can be used for sequencing or transcript quantifi...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Xingfa Han, Laura L. Burger, David Garcia-Galiano, Suzanne M. Moenter, Martin G. Myers, Jr, David P. Olson, Carol F. Elias
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2021-06-01
Schriftenreihe:	STAR Protocols
Schlagworte:	Gene Expression Neuroscience Protein Biochemistry
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S2666166721002963
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