Protocol to extract actively translated mRNAs from mouse hypothalamus by translating ribosome affinity purification

Summary: Here, we present an in-depth protocol for extracting ribosome-bound mRNAs in low-abundance cells of hypothalamic nuclei. mRNAs are extracted from the micropunched tissue using refined translating ribosome affinity purification. Isolated RNAs can be used for sequencing or transcript quantifi...

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Principais autores:	Xingfa Han, Laura L. Burger, David Garcia-Galiano, Suzanne M. Moenter, Martin G. Myers, Jr, David P. Olson, Carol F. Elias
格式:	Artigo
語言:	Inglês
出版:	Elsevier 2021-06-01
叢編:	STAR Protocols
主題:	Gene Expression Neuroscience Protein Biochemistry
在線閱讀:	http://www.sciencedirect.com/science/article/pii/S2666166721002963
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