A Cas9 with PAM recognition for adenine dinucleotides
Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting chimeras.
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| Auteurs principaux: | , , , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Portfolio
2020-05-01
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| Collection: | Nature Communications |
| Accès en ligne: | https://doi.org/10.1038/s41467-020-16117-8 |
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